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Clinical Chemistry 1: 234-240, 1955;
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Clinical Chemistry, Vol 1, 234-240, Copyright © 1955 by the American Association for Clinical Chemistry

Estimation of Hydrocortisone Secretion

Method of Calculation from Urinary-Excretion Data

Robert H. Silber 1

1 Merck Institute for Therapeutic Research, Rahway, N. J.

A method has been described for the estimation of the secretion of hydrocortisone by the adrenals of man, based exclusively upon urinary-excretion data. In an 8-hour period (8:30 A.M.-4:30 P.M.)5 subjects secreted an average of 13.8 mg. of hydrocortisone. In 1 subject ACTH was found to increase the adrenal output whereas aspirin, ascorbic acid, and Pyribenzamine had little, if any, effect.

The possible application of the approach to the measurement of the formation of other substances in the body has been noted.

Submitted on April 18, 1955






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Copyright © 1955 by the American Association for Clinical Chemistry.