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Clinical Chemistry 10: 942-949, 1964;
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Clinical Chemistry, Vol 10, 942-949, Copyright © 1964 by the American Association for Clinical Chemistry

Analysis on Heat Coagulated Blood and Serum

V. Determination of Glucose

Morris London 1 and Jesse H. Marymont Jr. 1

1 Division of Laboratories, North Shore Hospital, Manhasset, N. V.

A method for the determination of serum glucose employing a heat clot technic for the elimination of protein and other interfering substances is described. Serum (0.10 ml.) and absolute methyl alcohol (0.09 ml. delivered as six drops) are mixed in a shell vial and a clot is formed by immersion of the vial in a 60° water bath for 4 min. The clot is overlaid with 2.0 ml. of water and the container is placed in a 37° water bath for 45 min.; 0.50 ml. of the extract is added to 6.0 ml. of a glucose oxidase reagent and the solution placed in a 37° water bath for 30 min. Two drops of 4N HCI is added and the absorbance at 420 mµ measured. The serum glucose is calculated in the usual manner by comparing the absorbance of the unknown with that of a standard. The method is simple, reliable, and well suited for routine use in the clinical laboratory.

Submitted on March 7, 1963






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Copyright © 1964 by the American Association for Clinical Chemistry.