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Clinical Chemistry 10: 1105-1111, 1964;
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Clinical Chemistry, Vol 10, 1105-1111, Copyright © 1964 by the American Association for Clinical Chemistry

A Quantitative Assay for Urinary Porphobilinogen

Donald J. Moore 1 and Robert F. Labbe 1

1 Department of Pediatrics, University of Washington School of Medicine, Seattle, Washington, 98105. (R. F. L.)

A procedure has been developed for the rapid, quantitative determination of urinary porphobilinogen. Interfering substances are removed by selectively adsorbing the porphobilinogen with an ion-exchange resin using a batch method. After acid elution of the porphobilinogen, Ehrlich's reagent is added to produce the chromophore, which is then measured spectrophotometrically. Obtaining absorption at two wavelengths permits a differentiation of porphobilinogen from interfering reactants not removed by the resin treatment. This assay is relatively simple, dependable, highly reproducible for increased amounts, and more specific than the qualitative assay.

Submitted on December 12, 1963
Accepted on January 13, 1964




The following articles in journals at HighWire Press have cited this article:


Home page
 Clin. Chem.Home page
M. Roshal, J. Turgeon, and P. M. Rainey
Rapid Quantitative Method Using Spin Columns to Measure Porphobilinogen in Urine
Clin. Chem., February 1, 2008; 54(2): 429 - 431.
[Abstract] [Full Text] [PDF]


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Copyright © 1964 by the American Association for Clinical Chemistry.