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Clinical Chemistry 10: 116-127, 1964;
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Clinical Chemistry, Vol 10, 116-127, Copyright © 1964 by the American Association for Clinical Chemistry

Determination of Cholesterol by Means of Phloroglucinol in Acid Solution

Benjamin Sahagian 1 and Victor E. Levine 2

1 3616 Mohawk St., Lincoln, Neb.
2 Department of Biological Chemistry and Nutrition, The Creighton University School of Medicine, Omaha, Neb.

Cholesterol is determined by taking advantage of the fact that the digitonin moiety of cholesteryl digitonide is a glycoside containing in its molecular structure one molecule of xylose and four molecules of galactose, and therefore reacts with a sugar reagent, such as phloroglucinol in acid solution, to form a stable color complex yielding quantitative results when measured spectrophotometrically. The method agrees well with results obtained by the Schoenheimer and Sperry (8) procedure.

Submitted on July 10, 1962






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Copyright © 1964 by the American Association for Clinical Chemistry.