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Clinical Chemistry 10: 146-152, 1964;
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Clinical Chemistry, Vol 10, 146-152, Copyright © 1964 by the American Association for Clinical Chemistry

Determination of Phenylalanine in Blood and Urine

L. I. Woolf 1 and B. L. Goodwin 1

1 Department of the Regius Professor of Medicine, Radcliffe Infirmary, Oxford, England.

A modification is described of the method of LaDu and Michael for estimating the concentration of L-phenylalanine and L-tyrosine in the blood by oxidation with L-amino acid oxidase. The proteins of serum or plasma are removed in an ultra-filtration step that greatly reduces the nonspecific absorption, increasing the sensitivity and precision of the method. The ultrafiltrate is added to a composite reagent containing all other necessary components. The method can be applied to urine after removal of pigments with basic lead acetate.

Submitted on September 16, 1962






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Copyright © 1964 by the American Association for Clinical Chemistry.