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Clinical Chemistry 10: 412-416, 1964;
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Clinical Chemistry, Vol 10, 412-416, Copyright © 1964 by the American Association for Clinical Chemistry

Modern Methods for Determining Cerebrospinal Fluid Protein

Derek Watson 1

1 Division of Biochemistry, Department of Pathology, The Royal Women's Hospital, Carlton N. 3, Melbourne, Australia.

A simple and rapid micro method for the determination of albumin and total protein in cerebrospinal and other body fluids is presented. It is based on the UV spectrophotometric measurements of neutral and acid-alcohol-treated spinal fluid and is shown to be accurate and reproducible. Using spectrophotometry at 220 mµ and two other recently improved technics, 84 specimens of spinal fluid from infants were analyzed for total protein. The results obtained showed good agreement over the range 30-200 mg./100 ml.

Submitted on November 28, 1962






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Copyright © 1964 by the American Association for Clinical Chemistry.