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Clinical Chemistry 10: 429-432, 1964;
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Clinical Chemistry, Vol 10, 429-432, Copyright © 1964 by the American Association for Clinical Chemistry

Gas Chromatographic Determination of Lower Alcohols in Biologic Samples

Harold Lyons 1 and Jacquelyn Bard 1

1 Chemistry Department, Southwestern at Memphis, and the Department of Pathology, University of Tennessee, Memphis, Tenn.

A procedure is described for the simultaneous determination of methanol, ethanol, and isopropanol in biologic samples, using a low-cost gas chromatograph. A combination of the use of anhydrous calcium sulfate (Drierite) absorption of most of the water in the sample followed by an n-butanol extraction of the alcohols from the solid absorbent is employed. This extraction technic is efficient and affords good sensitivity while eliminating the need for hydrogen flame detection and sample-port clean-out.

Submitted on January 31, 1963






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Copyright © 1964 by the American Association for Clinical Chemistry.