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Clinical Chemistry 10: 519-532, 1964;
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Clinical Chemistry, Vol 10, 519-532, Copyright © 1964 by the American Association for Clinical Chemistry

Automated Determination of Serum Glutamic Oxalacetic and Glutamic Pyruvic Transaminase

Roscoe R. Schaffert 1, George R. Kingsley 1, and Gloria Getchell 1

1 Clinical Biochemistry Laboratory, Veterans Administration Center, and Department of Biological Chemistry, School of Medicine, University of California, Los Angeles 25, Calif.

The determination of serum glutamic oxalacetic and glutamic pyruvic transaminase has been simplified by the use of automated analysis. The flow diagrams, concentration of reagents, and calculation of the color unit in terms of the Karmen unit are shown for 2,4-dinitrophenylhydrazine and 6-benzamido-4-methoxy-m-toluidine diazonium chloride as the reacting dyes. Advantages of both methods are compared and discussed.

Submitted on October 25, 1962







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Copyright © 1964 by the American Association for Clinical Chemistry.