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Clinical Chemistry 10: 686-703, 1964;
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Clinical Chemistry, Vol 10, 686-703, Copyright © 1964 by the American Association for Clinical Chemistry

An Automated Procedure for the Simultaneous Determination of Calcium and Phosphorus

Gerald Kessler 1 and Morris Wolfman 1

1 Division of Neoplastic Medicine and Division of Medicine, Montefiore Hospital, Bronx 67, N. Y.

A completely automated method for the simultaneous or individual determination of calcium and phosphorus in serum and urine without preliminary sample treatment is reported. Separation of calcium and phosphorus from interfering materials is achieved by dialysis under acid conditions. The dialyzed calcium is determined colorimetrically using a metal complexing dye (cresolphthalein complexone) in an alkaline medium. Magnesium, phosphate, citrate, sulfate, etc., did not introduce significant errors under the test conditions. Inorganic phosphorus is determined by a modified Fiske-SubbaRow procedure. Reproducibility and recovery data as well as comparisons with manual methods are presented.

Submitted on July 12, 1963




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