Clinical Chemistry AACC Online Job Center
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 10: 704-720, 1964;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Radin, N.
Right arrow Articles by Gramza, A. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Radin, N.
Right arrow Articles by Gramza, A. L.

Clinical Chemistry, Vol 10, 704-720, Copyright © 1964 by the American Association for Clinical Chemistry

Differential Spectrophotometric Determination of Calcium

Nathan Radin 1 and Adalbert L. Gramza 1

1 Division of Biochemistry, Rochester General Hospital, Rochester 8, N. Y.

The appearance of kits for the determination of micro amounts of calcium stimulated our interest in the use of Eriochrome Blue SE for this analysis. A study of spectrophotometric curves indicates that, at pH values above 13.7, calcium will complex and cause a change of dye absorbance while magnesium does not complex with the dye. A differential spectrophotometric technic is described in this paper in which the spectrophotometer is set at zero absorbance with a dye-calcium standard solution or a dye-calcium sample solution as reference and the absorbance of the dye solution then measured. For a set of standards the absorbance-calcium relationship is linear. With the Beckman spectrophotometer, Model DU, it has been found that 1 part of serum to 100 parts of alkaline dye solution (100 lambda of serum to 10 ml. alkaline dye solution) can be used. The technic shows greater sensitivity and accuracy than do previous methods using Eriochrome Blue SE.

Submitted on October 22, 1962






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1964 by the American Association for Clinical Chemistry.