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Clinical Chemistry 11: 10-16, 1965;
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Clinical Chemistry, Vol 11, 10-16, Copyright © 1965 by the American Association for Clinical Chemistry

Micromethod for the Estimation of Plasma Fibrinogen

Robert G. Martinek 1 and Roland E. Berry 2

1 Division of Laboratories, Chicago Board of Health, Chicago, Ill.
2 Laboratory Service, Veterans Administration Hospital, Iron Mountain, Mich.

Fibrinogen is quantitatively precipitated from plasma by 1.20 M phosphate buffer. The amount of precipitated fibrinogen is determined turbidimetrically at 450 mµ. The method requires 0.25 ml. of plasma for the blank and sample. Reliability of the method has been established by comparison with an ammonium sulfate turbidimetric method and a colorimetric method, demonstration of specificity, variation in incubation temperature, demonstration of optimal molarity and pH, and replicate analyses.

Submitted on August 22, 1963






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Copyright © 1965 by the American Association for Clinical Chemistry.