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Clinical Chemistry 11: 29-36, 1965;
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Clinical Chemistry, Vol 11, 29-36, Copyright © 1965 by the American Association for Clinical Chemistry

The Basis for Elevation of Serum Glutamic Pyruvic Transaminase Levels in Serum Stored on Clots

W. B. Elliott 1 and Harold Rosamilia 1

1 Department of Biochemistry, School of Medicine, State University of New York at Buffalo and the Bacteriology Laboratory, Sisters of Charity Hospital, Buffalo 14, N. Y.

A modification of the serum glutamic-pyruvic transaminase colorimetric assay is described which reduces the error due to preformed keto acids, and thus permits evaluation of "corrected" transaminase levels in serum specimens that have stood on the clot up to three weeks at 4°. Evidence is presented that even in bacterially contaminated specimens the major cause of elevation in levels during storage is a rise in preformed keto acids.

Submitted on February 11, 1963
Accepted on April 29, 1964






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Copyright © 1965 by the American Association for Clinical Chemistry.