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Clinical Chemistry 11: 44-57, 1965;
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Clinical Chemistry, Vol 11, 44-57, Copyright © 1965 by the American Association for Clinical Chemistry

Air-Cooled Extractors and Their Use in Determination of Cholesterol

Frederic E. Holmes 1

1 47 Raymond Street, Falmouth, Mass.

For the purpose of determination of cholesterol in blood, serum, or other fluids of high cholesterol content, an extractor, previously described, has been devised which dries and extracts the sample on filter paper in one continuous operation with one solvent—chloroform. Moisture driven from the sample was collected above the refluxed solvent in a trap inserted between the water-cooled condenser and the sample. In order to avoid limiting the apparatus to location near a water outlet, and for greater ease and speed in handling the condensers, air cooling has since been substituted. A choice of several designs of trap is offered. Boiler-receiver tubes made to fit the Klett-Summerson photoelectric colorimeter provide for the extraction and the development and reading of the colors, all in the same tubes.

The method of extraction is given, and the errors due to esters of cholesterol and other substances are discussed.

Submitted on November 19, 1963
Accepted on March 16, 1964






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Copyright © 1965 by the American Association for Clinical Chemistry.