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Clinical Chemistry 11: 58-62, 1965;
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Clinical Chemistry, Vol 11, 58-62, Copyright © 1965 by the American Association for Clinical Chemistry

New Method for Determination of Alkylating Agents in Biologic Fluids

Yulin L. Tan 1 and Donald R. Cole 1

1 Surgical Research Laboratories, Department of Surgery, St. Vincent's Hospital of the City of New York, New York 11, N. Y., and New York University School of Medicine.

An accurate, reproducible colorimetric method for determining minute amounts of alkylating agents in biologic fluids has been developed. Utilizing this method, we have been able to detect less than 0.1 µg. of nitrogen mustard (HN2), or Thio-TEPA in 1 ml. of plasma, lymph, urine, or tissue homogenate. The biologic fluid was deproteinized with zinc sulfate, barium hydroxide, and methanol; the supernatant was used to alkylate gamma-(4-nitrobenzyl) pyridine (NBP) under optimum conditions. The color of the quaternary pyridinium NBP derivative was extracted into ethyl acetate and read on a Beckman DU spectrophotometer utilizing microcells.

Submitted on March 10, 1964
Accepted on May 29, 1964






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Copyright © 1965 by the American Association for Clinical Chemistry.