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Clinical Chemistry 11: 905-913, 1965;
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Clinical Chemistry, Vol 11, 905-913, Copyright © 1965 by the American Association for Clinical Chemistry

Determination of 3-Methoxy-4-Hydroxymandelic Acid (VMA) in Urine by Thin-Layer Chromatography

J. S. Annino 1, M. Lipson 1, and L. A. Williams 1

1 Massachusetts Memorial Hospitals, Boston University Medical Center, and Leary Laboratory, Boston, Mass. 02118.

From studies of several variables, a method has been developed for the separation and quantitation of 3-methoxy-4-hydroxymandelic acid (VMA) in urine by thin-layer chromatography. The urine is pretreated with acid and Florisil, and then extracted with ethyl acetate. Thin-layer chromatography is performed on silica gel with a butanol: acetic acid:water solution. The VMA spot is located by spraying with fast red GG and potassium carbonate. After removal from the plate, maximum color is developed and quantitated by reading in a spectrophotometer at 510 mµ.

Submitted on March 8, 1965
Accepted on June 30, 1965






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Copyright © 1965 by the American Association for Clinical Chemistry.