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Clinical Chemistry 11: 937-942, 1965;
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Clinical Chemistry, Vol 11, 937-942, Copyright © 1965 by the American Association for Clinical Chemistry

Evaluation of a Cellulose-Acetate Electrophoresis System for Serum Protein Fractionation

Alex Kaplan 1 and John Savory 2

1 University Hospital and Department of Biochemistry, University of Washington, Seattle, Wash. 98105.
2 postdoctoral trainee supported by Training Grant in Clinical Chemistry, T1 GM 776-01, from the National Institutes of Health.

A rapid system for the quantitative fractionation of serum proteins by electrophoresis on cellulose-acetate membranes was evaluated and found to be quite precise. The reproducibility (coefficient of variation) of the routine fractionation of a control serum carried out 40 times during a 15-week period was 2.4% for albumin and 14.2, 6.0, 6.1, and 5.2%, respectively, for the alpha1-, alpha2-, beta-, and gamma-globulin fractions.

Normal values are given for serum protein fractions (specimens from nonprofessional blood donors) obtained by cellulose acetate electrophoresis.

Submitted on April 10, 1965
Accepted on July 14, 1965




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Copyright © 1965 by the American Association for Clinical Chemistry.