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Clinical Chemistry 11: 951-958, 1965;
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Clinical Chemistry, Vol 11, 951-958, Copyright © 1965 by the American Association for Clinical Chemistry

Fluorometric Measurement of Creatine Kinase Activity

Sylvan M. Sax 1 and John J. Moore 1

1 Department of Pathology, The Western Pennsylvania Hospital, Pittsburgh, Pa. 15224.

The amount of creatine liberated in the creatine kinase reaction is conveniently measured by formation of a fluophor with ninhydrin in strongly alkaline solution. The method is rapid and sensitive. The incubation conditions developed are more nearly optimal than those in previous methods.

Submitted on May 20, 1965
Accepted on July 23, 1965




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Copyright © 1965 by the American Association for Clinical Chemistry.