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Clinical Chemistry 11: 1009-1018, 1965;
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Clinical Chemistry, Vol 11, 1009-1018, Copyright © 1965 by the American Association for Clinical Chemistry

Evaluation of Hydrolytic Products of 17-Ketosteroids by Means of Gas-Liquid Chromatography

L. P. Cawley 1, B. O. Musser 1, W. Faucette 1, S. Beckloff 1, and H. Learned 1

1 Division of Hormone Chemistry, Wesley Medical Research Foundation, Wesley Medical Center, Wichita 14, Kan.

In the course of evaluating urinary 17-ketosteroid (17-KS) gas-liquid chromatographic patterns, a number of peaks with retention times shorter than that of 17-KS were constantly observed. This background material was found to be degradation products of 17-KS produced by HCI hydrolysis. A study with pure 17-KS as a mixture and as individual steroids was used to identify the steroids responsible for most of the 9 compounds with retention times less than etiocholanolone. Hydrolysis in ethyl acetate containing perchloric acid produced smaller amounts of degradation products than HCI-hydrolysis. Enzymatic hydrolysis appears to be the most acceptable system for preparation of the extracts from urine for this system of assay.

Submitted on May 10, 1965
Accepted on July 26, 1965






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Copyright © 1965 by the American Association for Clinical Chemistry.