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Clinical Chemistry 11: 1058-1063, 1965;
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Clinical Chemistry, Vol 11, 1058-1063, Copyright © 1965 by the American Association for Clinical Chemistry

Electrophoretic Fractionation of Serum Glycoproteins on Cellulose Acetate

R. L. Kelsey 1, T. P. deGraffenried 1, and R. C. Donaldson 1

1 Departments of Clinical Pathology, deGraffenried-Fisher Laboratories, DeKalb, Ill., and Illinois Masonic Hospital, Chicago, Ill.

In a procedure for the routine electrophoretic fractionation of serum glycoproteins on cellulose acetate, electrophoretic fractionation is followed by staining utilizing the periodic acid-Schiff reaction. The patterns are scanned with a recording densitometer and simultaneously quantitated with an attached integrator. For 100 healthy blood donors,the means and standard deviations were: albumin, 10.9±1.4; alpha1,18.6±1.9; alpha2, 29.6±2.3; beta, 21.6±2.0; and gamma, 19.3±1.9%, respectively.

Submitted on May 5, 1965
Accepted on August 26, 1965






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Copyright © 1965 by the American Association for Clinical Chemistry.