Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 11: 1064-1067, 1965;
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Welch, R. M.
Right arrow Articles by Conney, A. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Welch, R. M.
Right arrow Articles by Conney, A. H.

Clinical Chemistry, Vol 11, 1064-1067, Copyright © 1965 by the American Association for Clinical Chemistry

A Simple Method for the Quantitative Determination of N-Acetyl-p-Aminophenol (APAP) in Urine

Richard M. Welch 1 and A. H. Conney 1

1 Wellcome Research Laboratories, Burroughs Wellcome & Co. (U.S.A.) Inc., Tuckahoe, N. Y., and the Department of Pharmacology, Albert Einstein College of Medicine, Bronx, N.Y.

A simple method is described for the rapid quantitative analysis of N-acetyl-p-amino-phenol (APAP) in urine. APAP and its conjugates present in the urine following the ingestion of acetophenetidin, APAP or acetanilid, are hydrolyzed with acid to p-aminophenol. This compound is coupled with phenol in the presence of hypobromite to form an indophenol dye whose concentration is determined spectrophotometrically. Application of this method to a study of 3 human subjects treated with acetophenetidin is described.

Submitted on July 19, 1965
Accepted on October 13, 1965






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1965 by the American Association for Clinical Chemistry.