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Clinical Chemistry 11: 91-96, 1965;
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Clinical Chemistry, Vol 11, 91-96, Copyright © 1965 by the American Association for Clinical Chemistry

A Micro Method for Serum Cholinesterase

Phil J. Garry 1 and Joseph I. Routh 1

1 Department of Biochemistry, College of Medicine, University of Iowa, Iowa City, Iowa.

A simple colorimetric method for determining serum or plasma cholinesterase has been described. The cholinesterase acts on acetylthiocholine to release thiocholine, which reacts with 5:5-dithiobis-(2-nitrobenzoic acid) to produce a yellow color. The activity is measured after incubation for 3 min. at 37° the reaction is stopped by the excess addition of quinidine sulfate. The difference in absorbance between the blank and test sample is then measured at 412 mµ. Duplicate determinations can be run on 0.1 ml. of serum or plasma, and the method is well suited to laboratories using routine procedures.

Submitted on May 25, 1964
Accepted on July 22, 1964




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