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Clinical Chemistry 11: 97-112, 1965;
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Clinical Chemistry, Vol 11, 97-112, Copyright © 1965 by the American Association for Clinical Chemistry

A Saccharogenic Method for Estimating Electrophoretic and Chromatographic Distribution of Human Serum Amylase

Ichiro Ujihira 1, Ronald L. Searcy 1, J. Edward Berk 1, and Shinichiro Hayashi 1

1 Departments of Medicine and Pathology, California College of Medicine and Los Angeles County General Hospital (Unit 2), Los Angeles 33, Calif.

The dinitrosalicylic acid reaction for measuring reducing sugar has been evaluated and found suitable for determining the saccharogenic activity of human serum. The sensitivity and reproducibility of the modified dinitrosalicylic acid method permits quantitation of the electrophoretic and chromatographic distribution of human serum amylase. Such fractionations suggest that a majority, if not all, saccharogenic activity in normal and certain abnormal specimens is chromatographically separable from other serum protein and exhibits an electrophoretic mobility similar to gamma-globulin.

Submitted on May 18, 1964
Accepted on August 26, 1964




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Copyright © 1965 by the American Association for Clinical Chemistry.