Clinical Chemistry AACC Online Job Center
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 11: 367-377, 1965;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Logan, J. E.
Right arrow Articles by Haight, D. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Logan, J. E.
Right arrow Articles by Haight, D. E.

Clinical Chemistry, Vol 11, 367-377, Copyright © 1965 by the American Association for Clinical Chemistry

Enzymatic Determination of Glucose in Urine by Automation Following Rapid Removal of Inhibitors by Ion-Exchange Resin

J. E. Logan 1 and D. E. Haight 1

1 Clinical Laboratories, Laboratory of Hygiene, Department of National Health and Welfare, Ottawa, Canada.

A commercial mixed-bed resin has been found effective for the rapid removal from urine of inhibitors of the glucose oxidase peroxidase system, thus allowing successful application of this specific method for glucose determination in urine specimens. The automated method of Hill and Kessler (10) for glucose determination was modified to make use of smaller quantities of the enzyme preparation and render the procedure less costly.

The percentage inhibition produced by graded amounts of uric acid and ascorbic acid was determined. Both compounds were found to be completely removed by the resin treatment. Normal levels of uric acid in urine inhibit the enzyme reaction markedly, whereas only large therapeutic doses of ascorbic acid would produce sufficient levels of this compound in urine for an inhibitory action.

Submitted on January 30, 1964
Accepted on July 29, 1964






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1965 by the American Association for Clinical Chemistry.