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Clinical Chemistry 11: 378-385, 1965;
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Clinical Chemistry, Vol 11, 378-385, Copyright © 1965 by the American Association for Clinical Chemistry

Determination of Microgram Quantities of Inorganic Boron in Mammalian Specimens

T. Konikowski 1 and Lee E. Farr 1

1 University of Texas M. D. Anderson Hospital and Tumor Institute, Houston, Texas 77025.

A method is presented for estimation of inorganic boron in mammalian tissues, blood, cerebrospinal fluid, and urine. The procedure is simple and rapid, qualifying it particularly to meet needs in clinical chemistry. The mammalian tissues are first enzymatically digested and extracted; then the deproteinized extract is allowed to react with quinalizarin in concentrated sulfuric acid. The intensity of the resultant color is read spectrophotometrically at 620 mµ. The boron concentration in the tissue analyzed is obtained from a standard reference graph for that tissue. The method is applicable to concentrations as low as I µg. of boron per gram of mammalian tissue or fluid.

Submitted on July 6, 1964
Accepted on September 20, 1964






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Copyright © 1965 by the American Association for Clinical Chemistry.