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Clinical Chemistry 11: 422-426, 1965;
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Clinical Chemistry, Vol 11, 422-426, Copyright © 1965 by the American Association for Clinical Chemistry

Method for Tissue Hemoglobin Analysis

Daniel Andersen 1 and William C. Shoemaker 2

1 Bispebjerg Post-Doctoral Fellow, Michael Reese Hospital.
2 Department of Surgical Research, Michael Reese Hospital and Medical Center, and the Department of Surgical Research, Hektoen Institute, Cook County Hospital, Chicago, Ill.

A colorimetric method for analysis of the red cell content of tissues is described, and compared with an independent radioassay method. The introduced method is based upon acid extraction of tissue, treatment with base, and adsorption of the remaining turbidity with lauryl sulfate. Preliminary data are presented on the reproducibility of the colorimetric method on multiple biopsies of the canine liver. Red cell content of biopsies obtained from a series of normal dogs was 0.031 ml./gm. of liver tissue with a standard deviation of ± 0.011. The red cell content of the liver of dogs in various physiologic conditions ranged up to 0.096 ml./gm. of liver tissue.

Submitted on July 16, 1964
Accepted on September 18, 1964






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Copyright © 1965 by the American Association for Clinical Chemistry.