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Clinical Chemistry 11: 441-447, 1965;
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Clinical Chemistry, Vol 11, 441-447, Copyright © 1965 by the American Association for Clinical Chemistry

Evaluation of a Dye-binding Method for Determination of Serum Albumin

Robert G. Martinek 1

1 4736 N. Tripp Ave., Chicago, Ill. 60630.

A simple dye-binding method for determining serum albumin, which employs the dye, 2-(4'-hydroxybenzeneazo) benzoic acid (HBABA), is described. Albumin in 0.1 ml. of serum is determined colorimetrically at 510 mµ. Reliability of the method has been established by comparison with another method using HBABA and with a salt fractionation-biuret method, by recovery experiments, variation in ambient temperature, demonstration of optimal dye molarity and pH, and replicate analyses.

Submitted on March 16, 1964
Accepted on September 15, 1964






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Copyright © 1965 by the American Association for Clinical Chemistry.