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Clinical Chemistry 11: 455-458, 1965;
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Clinical Chemistry, Vol 11, 455-458, Copyright © 1965 by the American Association for Clinical Chemistry

Microdetermination of Proline in Serum

G. K. Summer 1 and N. O. Roszel 1

1 Department of Pediatrics, School of Medicine, University of North Carolina, Chapel Hill, N. C. 27515.

An accurate method for determination of proline in serum has been adapted to a micro scale, with good results. Acidic ethanol is used as a serum deproteinizing agent prior to development of color with ninhydrin and its extraction into benzene. The range of accuracy is ± 3.0% with as little as 0.2 ml. serum. The microdetermination of this amino acid facilitates study of the intermediary metabolism of proline in pediatric patients as well as in small laboratory animals. The method is also useful in the study of amino acid and collagen biosynthesis in a number of experimental systems.

Submitted on August 24, 1964
Accepted on October 12, 1964






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Copyright © 1965 by the American Association for Clinical Chemistry.