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Clinical Chemistry 11: 465-470, 1965;
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Clinical Chemistry, Vol 11, 465-470, Copyright © 1965 by the American Association for Clinical Chemistry

Estimation of Urinary 5-Hydroxyindoleacetic Acid by Paper Chromatography-a Sensitive Method

Helen K. Berry 1 and Indira Krupanidhi 2

1 Children's Hospital Research Foundation and Department of Pediatrics, College of Medicine, University of Cincinnati, Cincinnati, Ohio, 45229.
2 World Health Organization Fellow, Government Medical College, Mysore, India.

A simple and highly sensitive method for the identification and quantitative estimation of 5-hydroxyindoleacetic acid (5-HIAA) concentration in urine is described. 5-HIAA was concentrated following extraction into ethyl acetate, and separated from other indolic acids by paper chromatography. A color reagent, p-dimethylaminocinnamaldehyde, was used to detect 5-HIAA on paper, in concentrations as low as 0.1 µg./cm.2 Urinary excretion levels could be measured below the normal range as well as in the normal range and above.

Submitted on July 7, 1964
Accepted on November 10, 1964






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Copyright © 1965 by the American Association for Clinical Chemistry.