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Clinical Chemistry, Vol 11, 521-526, Copyright © 1965 by the American Association for Clinical Chemistry
1 Section of Clinical Chemistry, Department of Pathology, University Hospital and Hillman Clinic, Birmingham, Ala. 35233.
Hydroxy Naphthol Blue as a metallochromic indicator in the chelometric measurement of calcium with EDTA provides a very distinct color change at the end-point in the titration of aqueous solutions, serum, and urine samples. Iron, magnesium, copper, phosphate, and bilirubin in concentrations normally encountered in serum and urine did not interfere with the reaction of the indicator. The results obtained with this indicator were not statistically different from those of serum samples analyzed by flame photometry or urine samples analyzed chelometrically with Cal-Red as the indicator.
Submitted on September 28, 1964
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