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Clinical Chemistry 11: 541-546, 1965;
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Clinical Chemistry, Vol 11, 541-546, Copyright © 1965 by the American Association for Clinical Chemistry

An Automated Procedure for Blood Phenylalanine

John B. Hill 1, George K. Summer 1, Murray W. Pender 1, and Norris O. Roszel 1

1 Departments of Pharmacology and Pediatrics, University of North Carolina, Chapel Hill, N. C.

The fluorometric analysis of phenylalanine described by McCaman and Robins has been adapted to automation. The procedure may be used on 20-µl. whole-blood samples which may be stored on filter paper for elution. The procedure is rapid, reproducible, and accurate, making it useful for screening large populations for phenylketonuria.

Submitted on November 10, 1964
Accepted on December 17, 1964




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