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Clinical Chemistry 11: 589-594, 1965;
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Clinical Chemistry, Vol 11, 589-594, Copyright © 1965 by the American Association for Clinical Chemistry

A Method for the Rapid Hydrolysis of Urinary Glucuronoside Metabolites of Testosterone

John R. Kent 1, Allen B. Rawitch 1, and Fredrick Peacock 1

1 Research Service, Wadsworth Veterans Administration Center, and the Department of Medicine, University of California Center for the Health Sciences, Los Angeles, Calif. 90804.

beta-glucuronidase hydrolysis of ethyl acetate-extracted glucuronoside metabolites of testosterone is more rapidly achieved than enzymatic hydrolysis of these conjugates in untreated urine. Less enzyme is required, provided the extracted conjugates are incubated under optimal conditions. Near-maximum hydrolysis required 24 hr. with the extracted conjugates and 72 hr. with untreated urine.

Submitted on October 26, 1964
Accepted on January 4, 1965






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Copyright © 1965 by the American Association for Clinical Chemistry.