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Clinical Chemistry 11: 617-623, 1965;
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Clinical Chemistry, Vol 11, 617-623, Copyright © 1965 by the American Association for Clinical Chemistry

The Determination of Thiamine in Small Amounts of Whole Blood and Serum by a Simplified Thiochrome Method

Than Myint 1 and Harold B. Houser 1

1 Nutrition Research Laboratory, Highland View Hospital, and the Departments of Preventive Medicine and Medicine, Western, Reserve University, Cleveland, Ohio.

This simplified thiochrome method for the determination of thiamine eliminates deproteinization, sample dilution (other than reagent), and purification by adsorption column; pH need not be adjusted as it is automatically controlled. The method depends upon the hydrolysis of whole blood and serum with N HCI, N/10 HCI, and diastase enzyme. Reproducibility was good; the mean differences (± S.D.) between duplicate blood and serum samples were 2.36 ± 2.87 and 1.5 ± 1.70 mµg./ml., respectively. Recovery of added thiamine ranged from 94 to 104% with a mean of 99.5 ± 3.41%. Storage of hydrolysates for 30 days did not change the results, and low serum concentrations could be measured in serum. Whole blood and serum values of thiamine in 44 healthy adults ranged from 11.3 to 47.8 mµg./ml. (mean, 29.3) and from trace amounts to 20.5 mµg./ml. (mean, 10.2), respectively.

Submitted on July 21, 1964
Accepted on January 28, 1965






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Copyright © 1965 by the American Association for Clinical Chemistry.