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Clinical Chemistry 11: 624-627, 1965;
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Clinical Chemistry, Vol 11, 624-627, Copyright © 1965 by the American Association for Clinical Chemistry

Automated and Manual Direct Methods for the Determination of Blood Urea

Walton H. Marsh 1, Benjamin Fingerhut 1, and Henry Miller 1

1 Department of Pathology, State University of New York, Downstate Medical Center, and the Institute of Pathology, Kings County Hospital Center, Brooklyn, N. Y. 11203.

Automated and manual direct methods for the determination of urea in blood or serum are described. These methods determine urea by the colored product formed when urea, in relatively weak acid solution, reacts with diacetyl monoxime in the presence of thiosemicarbazide and ferric ion. Results are compared with those obtained by urease conversion of urea to ammonia and measurement of the ammonia by nesslerization.

Submitted on January 16, 1964
Accepted on January 5, 1965




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 Arch OphthalmolHome page
E. J. Gralla and L. Rubin
Ocular Studies With Para-chlorophenylalanine in Rats and Monkeys
Arch Ophthalmol, June 1, 1970; 83(6): 734 - 740.
[Abstract] [PDF]

Home page
 Arch Intern MedHome page
I. H. Shehadeh, C. B. Carpenter, C. H. Monterio, and J. P. Merrill
Renal Allograft Rejection: An Analysis of Lysozymuria, Serum Complement, Lymphocyturia, and Heterophil Antibodies
Arch Intern Med, May 1, 1970; 125(5): 850 - 857.
[Abstract] [PDF]


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