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Clinical Chemistry 11: 628-632, 1965;
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Clinical Chemistry, Vol 11, 628-632, Copyright © 1965 by the American Association for Clinical Chemistry

Simplified Preparation of Blood Hemolysates for Hemoglobin Electrophoresis

R. Quentin Blackwell 1 and Jeanette Tung-Hsiang Huang 1

1 Department of Biochemistry, U. S. Naval Medical Research Unit No. 2, Taipei, Taiwan, Republic of China.

Hemolysates made directly from unwashed red cells or unwashed blood clots can be used successfully for screening populations for abnormal hemoglobins by starch-gel electrophoresis. The cells or clot, mixed with an equal volume of water, are frozen overnight or longer, and the resulting supernatant hemolysate used directly for electrophoresis. The simplified procedure has been used to identify several abnormal hemoglobins including A+E, A+H, A+J, and electrophoretically fast fetal hemoglobins. The principal advantages of the procedure include substantial economy in time and increased flexibility in sample collection and storage. The method is particularly useful with small blood samples collected without venipuncture.

Submitted on November 19, 1964
Accepted on January 14, 1965




The following articles in journals at HighWire Press have cited this article:


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R. Q. Blackwell, J. T.-H. Huang, and I. H. Ro
Hemoglobin Variants in Koreans: Hemoglobin G Taegu
Science, November 24, 1967; 158(3804): 1056 - 1057.
[Abstract] [PDF]

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R. Q. Blackwell, B.-N. Blackwell, J. T.-H. Huang, L.-C. Chien, A. Samaharn, C. Thephusdin, and C. Borvornsin
Hemoglobin JKorat in Thais
Science, December 17, 1965; 150(3703): 1614 - 1615.
[Abstract] [PDF]


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Copyright © 1965 by the American Association for Clinical Chemistry.