Clinical Chemistry AACC Online Job Center
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 11: 681-690, 1965;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Clarke, J. T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Clarke, J. T.

Clinical Chemistry, Vol 11, 681-690, Copyright © 1965 by the American Association for Clinical Chemistry

Purification and Analysis of Bilirubin

John T. Clarke 1

1 Children's Cancer Research Foundation and the Division of Laboratories and Research, The Children's Hospital Medical Center, Boston, Mass. 02115.

Pure crystalline bilirubin was isolated from human, calf, hog, and dog gallbladder bile and compared with commercially-available materials. All may be purified by recrystallization from pyridine. The extinction coefficient in chloroform is 60,000-61,000 at 450-453 mµ.

Standardization of the analytical "azobilirubin" (van den Bergh) reaction may be based on aqueous alkaline solutions of bilirubin.

Paper chromatography of azobilirubins derived from various sources of bilirubin showed identical patterns.

Submitted on December 15, 1964
Accepted on March 12, 1965






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1965 by the American Association for Clinical Chemistry.