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Clinical Chemistry 11: 691-699, 1965;
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Clinical Chemistry, Vol 11, 691-699, Copyright © 1965 by the American Association for Clinical Chemistry

Use of Bilirubin Standards

Samuel Meites 1 and John W. Traubert 1

1 Department of Pediatries, The Ohio State University College of Medicine, and the Clinical Chemistry Laboratory, The Children's Hospital, Columbus, Ohio 43205.

A stable stock solution of bilirubin in chloroform may be used to standardize quantitative determination of bilirubin in serum as "azobilirubin." The molar absorptivity of azobilirubin (560 mµ, 1 cm.) is 70,300 ±280 (mean ± 1 S.D.) when determined from chloroform-methanol standards treated with a modified van den Bergh reagent in the Malloy and Evelyn procedure for total bilirubin. The absorptivity of azobilirubin prepared from bilirubin in serum standards is essentially the same. The molar absorptivity of bilirubin in serum (460 mµ, 1 cm.) is 49,100 ±690 (mean± 1 S.D.). Manufacturers of standard bilirubin should use the molar constants as criteria of quality. Two of three preparations differed by 6.8 and 8.5% from the molar absorptivity found in standards made by the authors. Reconstituted commercial standards are stable for 3 days when stored at 5°.

Submitted on October 21, 1964
Accepted on April 7, 1965






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Copyright © 1965 by the American Association for Clinical Chemistry.