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Clinical Chemistry 11: 700-707, 1965;
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Clinical Chemistry, Vol 11, 700-707, Copyright © 1965 by the American Association for Clinical Chemistry

A Coulometric Determination of Urea Nitrogen in Blood and Urine

Gary D. Christian 1, Edward C. Knoblock 1, and William C. Purdy 1

1 Division of Biochemistry, Walter Reed Army Intitute of Research, Walter Reed Army Medical Center, Washington, D. C. 20012, and the Department of Chemistry, University of Maryland, College Park, Md. 20742.

To determine urea nitrogen, ammonia resulting from urease hydrolysis of urea is titrated with coulometrically generated hypobromite ion using a direct amperometric end-point detection. In blood samples, the ammonia is first separated by the microdiffusion technic. The ammonia may be titrated directly in urine samples.

Submitted on August 24, 1964
Accepted on March 5, 1965






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Copyright © 1965 by the American Association for Clinical Chemistry.