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Clinical Chemistry 11: 716-728, 1965;
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Clinical Chemistry, Vol 11, 716-728, Copyright © 1965 by the American Association for Clinical Chemistry

Determination of Serum Calcium by Means of Sodium Alizarinsulfonate

Harold V. Connerty 1 and Anglis R. Briggs 1

1 Pathology and Laboratory Service, Veterans Administration Hospital, Wilmington, Del.

A direct method is described for the determination of serum calcium, in which reaction with sodium alizarin sulfonate takes place under optimal conditions to produce a colored complex of calcium, the absorbance of which is read at 550 mµ. One variation in procedure, in which prior isolation of the calcium as the insoluble oxalate is made, is also described.

Submitted on June 17, 1964
Accepted on April 26, 1965






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Copyright © 1965 by the American Association for Clinical Chemistry.