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Clinical Chemistry 11: 748-762, 1965;
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Clinical Chemistry, Vol 11, 748-762, Copyright © 1965 by the American Association for Clinical Chemistry

An Automated Fluorometric Method for Determination of Lactic Dehydrogenase in Serum

Lewis Brooks 1 and Harry G. Olken 1

1 Clinical Laboratory, Lynn Hospital, Lynn, Mass.

A simple automated method for the determination of lactic dehydrogenase (LDH) activity in serum by the forward lactate rarr pyruvate reaction is presented. Enzyme activity is determined by measuring the fluorescence of DPNH formed after a fixed incubation time. Detailed studies of various parameters of the reaction, reproducibility, and recovery data, and a comparison with two manual methods for LDH are presented.

Submitted on November 27, 1964
Accepted on May 7, 1965




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Copyright © 1965 by the American Association for Clinical Chemistry.