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Clinical Chemistry 11: 840-845, 1965;
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Clinical Chemistry, Vol 11, 840-845, Copyright © 1965 by the American Association for Clinical Chemistry

Determination of Glucose in Biologic Fluids with an Automated Enzymatic Procedure

Michael Robin 1 and Abraham Saifer 1

1 Biochemistry Department, Isaac Albert Research Institute of the Jewish Chronic Disease Hospital, Brooklyn 3, N. Y.

An automated procedure for the enzymatic determination of "true" glucose with the glucose oxidase-peroxidase system is described. A threefold increase in sensitivity was obtained through measurement of the stable, pink chromogen obtained at high acid concentration. Good reproducibility and stoichiometry was obtained over the range of 50-400 mg./l00 ml. at a sampling rate of 40/hr. The normal range (70-105 mg./100 ml.) and the error of the method (±2.0%) are about the same as that obtained with the manual method of Saifer and Gerstenfeld (7).

Submitted on March 19, 1965
Accepted on May 22, 1965






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Copyright © 1965 by the American Association for Clinical Chemistry.