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Clinical Chemistry 11: 846-854, 1965;
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Clinical Chemistry, Vol 11, 846-854, Copyright © 1965 by the American Association for Clinical Chemistry

Comparison of Two Current Colorimetric Procedures for Determination of Serum Glutamic Oxaloacetic Transaminase

R. M. Nakamura 1, R. Magsaysay 2, and G. M. Kunitake 3

1 Division of Experimental Pathology, Scripps Clinic and Research Foundation, La Jolla, Calif. 92037.
2 Departments of Pathology, Los Angeles County Harbor General Hospital, Torrance, Calif., and University of California Medical Center, Los Angeles 24, Calif.
3 Departments of Pathology, Los Angeles County Harbor General Hospital, Torrance, Calif., and University of California Medical center, Los Angeles 24, Calif.

A comparison of two colorimetric procedures for the determination of serum glutamic oxaloacetic acid (SGOT) showed that both the dinitrophenylhydrazine and diazonium salt colorimetric assay procedures have good precision and reproducibility acceptable to most clinical laboratories. Babson's diazonium salt procedure (3) definitely shows greater precision in the important borderline range of values than the dinitrophenylhydrazine method based on the Reitman-Frankel method (2). Advantages and disadvantages of both procedures are discussed.

Submitted on April 7, 1965
Accepted on May 28, 1965






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Copyright © 1965 by the American Association for Clinical Chemistry.