Clinical Chemistry
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Clinical Chemistry 11: 855-861, 1965;
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Clinical Chemistry, Vol 11, 855-861, Copyright © 1965 by the American Association for Clinical Chemistry

Determination of Calcium in Urine by the Chloranilate Method

William W. Webster 1 and William H. Kern 1

1 Clinical Laboratory Medical Group, and the Department of Pathology, The Hospital of time Good Samaritan, Los Angeles, Calif.

Evidence is presented that the colorimetric chloranilate calcium method can be accurately applied to urine provided that elimination of interfering substances, which include magnesium, is performed prior to the determination. Isolation of calcium as the oxalate, followed by perchloric acid digestion, has been shown to be an acceptable method whereby urinary calcium can be put in a suitable state for analysis. Allowing the urine to stand for 1 hr. at pH 2 prior to the oxalate precipitation has been shown to be a simple means of eliminating the sampling error due to calcium precipitates.

Submitted on April 10, 1965
Accepted on May 29, 1965






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Copyright © 1965 by the American Association for Clinical Chemistry.