Clinical Chemistry Link to Randox Laboratories Web Site
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 11: 862-868, 1965;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fingerhut, B.
Right arrow Articles by Marsh, W. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fingerhut, B.
Right arrow Articles by Marsh, W. H.

Clinical Chemistry, Vol 11, 862-868, Copyright © 1965 by the American Association for Clinical Chemistry

A Rapid Saccharogenic Method for the Determination of Serum Amylase

Benjamin Fingerhut 1, Rocco Ferzola 1, Alfred Poock 1, and Walton H. Marsh 1

1 Institute of Pathology, Kings Country Hospital Center, and Department of Pathology, State University of New York, Downstate Medical Center, Brooklyn, N. Y. 11203.

In this procedure for serum amylase the sugars formed by enzymatic starch hydrolysis are quantitatively measured in a protein-free tungstic acid filtrate by the alkaline reduction of ferricyanide to ferrocyanide. The ferrocyanide formed is readily determined by measurement of the color produced with phosphomolybdic acid at room temperature.

The reducing sugar formed, expressed as milligrams per hundred milliliters of glucose measured by alkaline ferricyanide reduction, was comparable in amount after 15 min. incubation at 37.5° to that formed at 30 min. as measured by reduction of alkaline copper reagent.

The values for 54 apparently healthy blood donors, given by the 15-min. incubation ferricyanide technic, ranged from 24-195 U., with a mean and standard deviation from the mean of 103 ± 35 U. With the conventional 30-min. incubation alkaline copper method, a mean and standard deviation from the mean of 101 ± 36 U. was obtained. Amylase values given by both procedures for serums in the normal and abnormal ranges are presented. The reproducibility and some of the factors affecting the sensitivity of the ferricyanide method have also been determined.

Submitted on December 9, 1964
Accepted on March 17, 1965






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1965 by the American Association for Clinical Chemistry.