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Clinical Chemistry 11: 876-888, 1965;
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Clinical Chemistry, Vol 11, 876-888, Copyright © 1965 by the American Association for Clinical Chemistry

An Automated p-Nitrophenylphosphate Serum Alkaline Phosphatase Procedure for the AutoAnalyzer

Stanley Morgenstern 1, Gerald Kessler 2, Joseph Auerbach 1, Richard V. Flor 1, and Bernard Klein 1

1 Automation Research Laboratory, Veterans Administration Hospital, Bronx, N. Y. 10468, and the Division of Neoplastic Medicine, Montefiore Hospital, Bronx, N. Y.
2 Bio-Science Laboratories, Los Angeles, Calif. 90025

A new and simplified procedure for the automated determination of serum alkaline phosphatase uses the AutoAnalyzer. The substrate p-nitrophenylphosphate in 2-amino-2-methyl-1-propanol offers the prime advantage of providing directly its own chromogen, p-nitrophenol, following enzyme action. The procedure also permits use of small sample volumes, provides linear reaction rates, and has a simple manifold design. Correlations are presented between the manual procedure and the automated system.

Submitted on March 3, 1965
Accepted on June 17, 1965




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Copyright © 1965 by the American Association for Clinical Chemistry.