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Clinical Chemistry 11: 889-897, 1965;
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Clinical Chemistry, Vol 11, 889-897, Copyright © 1965 by the American Association for Clinical Chemistry

An Automated p-Nitrophenylphosphate Serum Alkaline Phosphatase Procedure for the "Robot Chemist"

Stanley Morgenstern 1, Gerald Kessler 2, Joseph Auerbach 3, Richard V. Flor 3, and Bernard Klein 3

1 Automation Research Laboratory, Veterans Administration Hospital, Bronx, N. Y. 10468, and the Division of Neoplastic Medicine, Montefiore Hospital, Bronx, N. Y.
2 Bio-Science Laboratories, Los Angeles, Calif. 90025.
3 Automation Research Laboratory, Veterans Administration Hospital, Broiix, N. Y. 10468, and the Division of Neoplastic Medicine, Montefiore Hospital, Bronx, N. Y.

A new, precise, and accurate procedure is described for the automated determination of serum alkaline phosphatase on the Robot Chemist. The substrate, p-nitrophenyl-phosphate in 2-amino-2-methyl-1-propanol, offers the prime advantage of providing directly its own chromogen, p-nitrophenol, following enzyme action. Other advantages are the small sample volumes required and linear reaction rates. Correlations are presented between the manual procedure and those used with both the Technicon Auto-Analyzer and the Robot Chemist.

Submitted on March 17, 1965
Accepted on June 23, 1965




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Copyright © 1965 by the American Association for Clinical Chemistry.