Clinical Chemistry Link to Randox Laboratories Web Site
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 12: 18-24, 1966;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Caraway, W. T.
Right arrow Articles by Marable, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Caraway, W. T.
Right arrow Articles by Marable, H.

Clinical Chemistry, Vol 12, 18-24, Copyright © 1966 by the American Association for Clinical Chemistry

Comparison of Carbonate and Uricase-Carbonate Methods for the Determination of Uric Acid in Serum

Wendell T. Caraway 1 and Herman Marable 1

1 Chemistry Laboratory, McLaren General Hospital, Flint, Mich.

A colorimetric carbonate procedure for the determination of uric acid has been modified to include incubation of serum with uricase to destroy uric acid. Residual nonurate chromogens are subtracted from total chromogens to obtain the concentration of "true" uric acid. Result obtained by the carbonate and the uricase-carbonate methods were in good agreement. The mean concentration of nonurate chromogens in serum is approximately 2% of the true uric acid values. Recovery of uric acid added to serum is essentially quantitative. Formaldehyde markedly inhibits uricase activity and interferes with recoveries. The uricase-carbonate method is applicable to hemolytic serum and to serums from patients with uremia or those receiving high doses of salicylate in which excess concentrations of nonurate chromogens may be encountered.

Submitted on May 7, 1964
Accepted on October 27, 1965






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1966 by the American Association for Clinical Chemistry.