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Clinical Chemistry 12: 670-680, 1966;
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Clinical Chemistry, Vol 12, 670-680, Copyright © 1966 by the American Association for Clinical Chemistry

Estrogens in Human Pregnancy Plasma

I. Studies with Gas Chromatography

Henry S. Kroman 1, Sheldon R. Bender 1, Albert N. Brest 1, Mark L. Moskovitz 1, and Mark O. King 1

1 Katz Research Laboratory, Section of Vascular Diseases and Renology, Hahnemann Medical College and Hospital, Philadelphia, Pa. 19102.

The concentration of the 3 classical estrogens, 17beta-estradiol, estrone, and estriol, was determined in pooled pregnancy plasma by a combination of chemical extraction and gas chromatography. When pregnancy plasma was fractionated by gas chromatography, peaks representing the 3 estrogens were characterized by melting point determination, infrared analysis, two-dimensional thin-layer chromatography, and formation of the acetate derivatives with subsequent gas chromatographic separation.

The mean concentrations of estrogens per 100 ml. of pregnancy plasma were: estrone, 7.65 ± 1.60 µg.; 17beta-estradiol, 3.39 ± 1.95 µg.; and estriol, 8.48 ± 2.34 µg.

Submitted on January 12, 1966
Accepted on March 4, 1966




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Copyright © 1966 by the American Association for Clinical Chemistry.