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Clinical Chemistry 12: 739-747, 1966;
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Clinical Chemistry, Vol 12, 739-747, Copyright © 1966 by the American Association for Clinical Chemistry

Determination of Total and Esterified Cholesterol in Serum

Charles Sobel 1 and Alberto Fernandez 1

1 Bio-Science Laboratories, 7600 Tyrone Ave., Van Nuys, Calif. 91405.

A simple method for the determination of total and esterified cholesterol is described. Serum lipids are extracted into a solvent mixture and aliquots of the extract are analyzed directly for free and total cholesterol by the Zlatkis reaction. Esterified cholesterol is calculated by difference. Free cholesterol is determined under conditions of temperature and solvent composition such that the cholesterol esters do not react. The method yields quantitative recoveries of free and esterified cholesterol from serum. Data are presented on the precision of the method, and on results of a comparison with the reference procedure of Sperry and Webb.

Submitted on May 17, 1966
Accepted on June 9, 1966






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Copyright © 1966 by the American Association for Clinical Chemistry.