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Clinical Chemistry 12: 767-770, 1966;
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Clinical Chemistry, Vol 12, 767-770, Copyright © 1966 by the American Association for Clinical Chemistry

Specificity of the Free Fatty Acid Titration by the Method of Dole, Modified According to Trout

J. Parijs 1, F. Barbier 1, and A. Elewaut 1

1 State University of Ghent, Policlinic for Internal Medicine, Akademisch Ziekenhuis, 115, De Pintelaan, Ghent, Belgium.

The specificity of free fatty acid (FFA) titration is significantly enhanced when the modification of Trout (5) is used and the heptane extract washed with dilute sulfuric acid. Lactate, pyruvate, acetate, beta-hydroxybutyrate, alpha-ketoglutarate, citrate, and urate, in concentrations found in normal human plasma, will not interfere with the titration. It can be expected that in some exceptional pathologic conditions, lactate, pyruvate, acetate, beta-hydroxybutyrate, and urate will not be completely removed from the heptane extract. Salicylate seems to cause considerable interference. Trout's procedure substantially reduces the interference caused by phospholipids.

Submitted on March 2, 1966
Accepted on June 21, 1966






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Copyright © 1966 by the American Association for Clinical Chemistry.