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Clinical Chemistry 12: 47-57, 1966;
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Clinical Chemistry, Vol 12, 47-57, Copyright © 1966 by the American Association for Clinical Chemistry

Direct Measurement of Serum Iron and Binding Capacity

Jesse F. Goodwin 1, Bramlett Murphy 1, and Marcel Guillemette 1

1 Core Laboratory, Clinical Research Center, Children's Hospital, and Wayne State University School of Medicine, Detroit, Mich.

Serum iron may be directly measured without protein precipitation, by incubation in an acetate buffer in the presence of a reducing agent and sulfonated bathophenanthro line. Mercaptoacetic and ascorbic acids have proved to be suitable reductants. Values obtained for serum iron by this procedure show excellent correlation with a trichloro acetic acid precipitation technic. Bilirubin interference may be compensated for. Hemoglobin interference is significant at values above 30 mg./100 ml. Lipid interference is not significant at concentrations below 1,960 mg./100 ml. The iron chelators, ethylenediaminetetraacetic acid (EDTA), diethylenetriaminepentaacetic acid (DTPA), and desferrioxamine do not interfere.

Both unsaturated iron-binding capacity (UIBC) and total iron-binding capacity (TIBC) may be estimated; values for both show good correlation. Serum iron, TIBC, UIBC, and % saturation values obtained from a group of children aged 4-12 years are presented. The advantages of the direct technic for serum iron, TIBC, and UIBC are simplicity and sensitivity.

Submitted on March 22, 1965
Accepted on October 26, 1965




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Copyright © 1966 by the American Association for Clinical Chemistry.